Roma dropped two points at home against Sassuolo last week, thus failing to catch Inter in the table and keep pace with Napoli and Juventus.
Atalanta beat Napoli in the Coppa Italia in midweek to progress to the semi-finals despite being underdogs.
This is a difficult match for Roma as Atalanta fear no team. Roma may drop points for the second weekend in a row.
Cagliari upset Atalanta in the last round and are now five points clear of the bottom three.
Juventus are on a three-game winning streak in the league and dominated Torino in the Coppa Italia on Wednesday. Now they will look for one final win before the break.
The hosts would be happy with a draw against Juventus but that's unlikely to happen here.